Molecular cloning and expression of Treponema pallidum DNA in Escherichia coli K-12.

A gene bank of Treponema pallidum DNA in Escherichia coli K-12 was constructed by cloning SauI-cleaved T. pallidum DNA into the cosmid pHC79. Sixteen of 800 clones investigated produced one or more antigens that reacted with antibodies from syphilitic patients. According to the separation pattern of...

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Bibliographic Details
Main Authors: van Embden, J D, van der Donk, H J, van Eijk, R V, van der Heide, H G, de Jong, J A, van Olderen, M F, Osterhaus, A B, Schouls, L M
Format: Artigo
Language:Inglês
Published: 1983
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC264541/
https://ncbi.nlm.nih.gov/pubmed/6311744
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