Molecular cloning and expression of Treponema pallidum DNA in Escherichia coli K-12.

A gene bank of Treponema pallidum DNA in Escherichia coli K-12 was constructed by cloning SauI-cleaved T. pallidum DNA into the cosmid pHC79. Sixteen of 800 clones investigated produced one or more antigens that reacted with antibodies from syphilitic patients. According to the separation pattern of...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	van Embden, J D, van der Donk, H J, van Eijk, R V, van der Heide, H G, de Jong, J A, van Olderen, M F, Osterhaus, A B, Schouls, L M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1983
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264541/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6311744/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.42.1.187-196.1983
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