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Comparison of a Commercial Real-Time PCR Assay for tcdB Detection to a Cell Culture Cytotoxicity Assay and Toxigenic Culture for Direct Detection of Toxin-Producing Clostridium difficile in Clinical Samples

Rapid detection of toxin-producing strains of Clostridium difficile is essential for optimal management of patients with C. difficile infection. The BD GeneOhm (San Diego, CA) Cdiff assay, a real-time PCR assay that amplifies tcdB, was compared to a cell culture neutralization assay (Wampole C. diff...

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Detalhes bibliográficos
Main Authors: Stamper, Paul D., Alcabasa, Romina, Aird, Deborah, Babiker, Wisal, Wehrlin, Jennifer, Ikpeama, Ijeoma, Carroll, Karen C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2643696/
https://ncbi.nlm.nih.gov/pubmed/19073875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01613-08
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