Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetri...

Disgrifiad llawn

Manylion Llyfryddiaeth

Cyhoeddwyd yn:	J Clin Microbiol
Prif Awduron:	Rotbart, H A, Sawyer, M H, Fast, S, Lewinski, C, Murphy, N, Keyser, E F, Spadoro, J, Kao, S Y, Loeffelholz, M
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	American Society for Microbiology (ASM) 1994
Pynciau:	Research Article
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264111/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7814507/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.32.10.2590-2592.1994
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!