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Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetri...

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Publicado no:J Clin Microbiol
Principais autores: Rotbart, H A, Sawyer, M H, Fast, S, Lewinski, C, Murphy, N, Keyser, E F, Spadoro, J, Kao, S Y, Loeffelholz, M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1994
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264111/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7814507/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.32.10.2590-2592.1994
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