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Nested PCR optimized for detection of Bordetella pertussis in clinical nasopharyngeal samples.

Several genes and sequences in Bordetella pertussis have been used as targets in diagnostic PCR assays. A previously developed single-step PCR assay for the detection of B. pertussis was based on an insertion sequence, IS480, that is present in about 70 to 80 copies in each genome. The diagnostic se...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Bäckman, A, Johansson, B, Olcén, P
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264099/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7814495/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.32.10.2544-2548.1994
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