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Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression

High-throughput screening (HTS) assays used in drug discovery frequently use reporter enzymes such as firefly luciferase (FLuc) as indicators of target activity. An important caveat to consider, however, is that compounds can directly affect the reporter, leading to nonspecific but highly reproducib...

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Detalhes bibliográficos
Main Authors: Auld, Douglas S., Thorne, Natasha, Maguire, William F., Inglese, James
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2638738/
https://ncbi.nlm.nih.gov/pubmed/19208811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0813345106
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