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Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression
High-throughput screening (HTS) assays used in drug discovery frequently use reporter enzymes such as firefly luciferase (FLuc) as indicators of target activity. An important caveat to consider, however, is that compounds can directly affect the reporter, leading to nonspecific but highly reproducib...
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| Główni autorzy: | , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
National Academy of Sciences
2009
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2638738/ https://ncbi.nlm.nih.gov/pubmed/19208811 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0813345106 |
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