Ab initio construction of a eukaryotic transcriptome by massively parallel mRNA sequencing

Defining the transcriptome, the repertoire of transcribed regions encoded in the genome, is a challenging experimental task. Current approaches, relying on sequencing of ESTs or cDNA libraries, are expensive and labor-intensive. Here, we present a general approach for ab initio discovery of the comp...

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Detalhes bibliográficos
Main Authors: Yassour, Moran, Kaplan, Tommy, Fraser, Hunter B., Levin, Joshua Z., Pfiffner, Jenna, Adiconis, Xian, Schroth, Gary, Luo, Shujun, Khrebtukova, Irina, Gnirke, Andreas, Nusbaum, Chad, Thompson, Dawn-Anne, Friedman, Nir, Regev, Aviv
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2009
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2638735/
https://ncbi.nlm.nih.gov/pubmed/19208812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0812841106
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