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Optimizing the Precision for Localizing Fluorescent Proteins in Living Cells by 2D Gaussian Fitting of Digital Images: Application to COPII-Coated Endoplasmic Reticulum Exit Sites

An insight into the operation of molecular motors has already been obtained under in vitro conditions from single-molecule tracking of proteins. It remains to analyze the effects of these motors on the position and secretion of specific organelles in the environment of the cell. For this purpose, we...

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Main Authors: Spence, Peter, Gupta, Vijay, Stephens, David J., Hudson, Andrew J.
格式: Artigo
語言:Inglês
出版: 2008
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635534/
https://ncbi.nlm.nih.gov/pubmed/18504570
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00249-008-0343-7
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