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pBT, a novel vector for tetracycline-regulated yeast three-hybrid assay

A novel yeast three-hybrid (Y3H) vector pBT was developed, which contains a tetracycline (Tet)-sensitive transactivator (tTA) expression unit and a Tet-responsive element (TRE)-driven 3rd protein expression unit within a single plasmid. To optimize tTA expression levels, several promoters for drivin...

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Detalhes bibliográficos
Autor principal: Moriyoshi, Koki
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2632908/
https://ncbi.nlm.nih.gov/pubmed/19050013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn969
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