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DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cere...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2632897/ https://ncbi.nlm.nih.gov/pubmed/19074487 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn991 |
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