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Microscale Solution Isoelectric Focusing as an Effective Strategy Enabling Containment of Hemeoglobin-Derived Products for High-Resolution Gel-Based Analysis of the Plasmodium falciparum Proteome

The high hemeozoin (beta-hemeatin) content of Plasmodium falciparum lysates imposes severe limitations on the analysis of the malarial proteome, in particular compromising the loading capacities of two-dimensional gels. Here we report on the adaptation of a recently developed solution-phase isoelect...

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Detalhes bibliográficos
Main Authors: Nirmalan, Niroshini, Flett, Fiona, Skinner, Tom, Hyde, John E., Sims, Paul F. G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2632839/
https://ncbi.nlm.nih.gov/pubmed/17696383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr070278r
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