Increased proteome coverage by combining PAGE and peptide isoelectric focusing: Comparative study of gel-based separation approaches

The in-depth analysis of complex proteome samples requires fractionation of the sample into subsamples prior to LC-MS/MS in shotgun proteomics experiments. We have established a 3D workflow for shotgun proteomics that relies on protein separation by 1D PAGE, gel fractionation, trypsin digestion, and...

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Bibliographic Details
Published in:Proteomics
Main Authors: Atanassov, Ilian, Urlaub, Henning
Format: Artigo
Language:Inglês
Published: BlackWell Publishing Ltd 2013
Subjects:
Technology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4285200/
https://ncbi.nlm.nih.gov/pubmed/23943586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201300035
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