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Cross-strand split tetra-Cys motifs as structure sensors in a β-sheet protein

We have designed ‘split tetra-Cys motifs’ that bind the biarsenical fluorescein dye FlAsH across strands of a model β-rich protein. Our strategy was to divide the linear FlAsH-binding tetra-Cys sequence such that dye could be fully liganded only when the strands were arranged in space correctly by n...

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Detalhes bibliográficos
Main Authors: Krishnan, Beena, Gierasch, Lila M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2631173/
https://ncbi.nlm.nih.gov/pubmed/18940670
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2008.09.006
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