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Real-Time Monitoring of Human Enterovirus (HEV)-Infected Cells and Anti-HEV 3C Protease Potency by Fluorescence Resonance Energy Transfer

A real-time assay system that allows monitoring of intracellular human enterovirus (HEV) protease activity was established using the principle of fluorescence resonance energy transfer (FRET). It was accomplished by engineering cells to constitutively express a genetically encoded FRET probe. The FR...

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Hlavní autoři: Tsai, Meng-Tian, Cheng, Yun-Hsiang, Liu, Yu-Ning, Liao, Nien-Chien, Lu, Wen-Wen, Kung, Szu-Hao
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2630644/
https://ncbi.nlm.nih.gov/pubmed/19015331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00841-08
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