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Detection of the pathogenic parasite Toxoplasma gondii by specific amplification of ribosomal sequences using comultiplex polymerase chain reaction.

Amplification of DNA sequences from ribosomal DNA (rDNA) was tested as a specific and sensitive method for the detection of small numbers of Toxoplasma gondii tachyzoite cells. We applied the polymerase chain reaction (PCR) on the basis of detection of the 110-fold repetitive rDNA as a target by usi...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Guay, J M, Dubois, D, Morency, M J, Gagnon, S, Mercier, J, Levesque, R C
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC262736/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8432803/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.2.203-207.1993
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