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Detection of the pathogenic parasite Toxoplasma gondii by specific amplification of ribosomal sequences using comultiplex polymerase chain reaction.

Amplification of DNA sequences from ribosomal DNA (rDNA) was tested as a specific and sensitive method for the detection of small numbers of Toxoplasma gondii tachyzoite cells. We applied the polymerase chain reaction (PCR) on the basis of detection of the 110-fold repetitive rDNA as a target by usi...

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Autors principals: Guay, J M, Dubois, D, Morency, M J, Gagnon, S, Mercier, J, Levesque, R C
Format: Artigo
Idioma:Inglês
Publicat: 1993
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC262736/
https://ncbi.nlm.nih.gov/pubmed/8432803
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