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Design and Evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified ribosomal DNA (rDNA) is routinely used to compare levels of diversity of microbial communities and to monitor population dynamics. While using PCR-DGGE to examine the bacteria in wine fermentations, we noted that several commonly used P...

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Udgivet i:Appl Environ Microbiol
Principais autores: Lopez, Isabel, Ruiz-Larrea, Fernanda, Cocolin, Luca, Orr, Erica, Phister, Trevor, Marshall, Megan, VanderGheynst, Jean, Mills, David A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2003
Fag:
Microbial Ecology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC262258/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602643/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.11.6801-6807.2003
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