Design and Evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified ribosomal DNA (rDNA) is routinely used to compare levels of diversity of microbial communities and to monitor population dynamics. While using PCR-DGGE to examine the bacteria in wine fermentations, we noted that several commonly used P...

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Detalhes bibliográficos
Main Authors: Lopez, Isabel, Ruiz-Larrea, Fernanda, Cocolin, Luca, Orr, Erica, Phister, Trevor, Marshall, Megan, VanderGheynst, Jean, Mills, David A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Microbial Ecology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC262258/
https://ncbi.nlm.nih.gov/pubmed/14602643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.11.6801-6807.2003
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