Cosmid cloning of Rickettsia prowazekii antigens in Escherichia coli K-12.

Rickettsia prowazekii DNA was partially digested with Sau3A or HindIII, ligated with the cosmid vector pHC79, packaged in vitro, and transduced into Escherichia coli HB101. Cosmid cloning of Sau3A-digested rickettsial DNA yielded 1,288 ampicillin-resistant colonies; 798 cosmid clones resulted with H...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Krause, D C, Winkler, H H, Wood, D O
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC261491/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2981194/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.47.1.157-165.1985
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