Cosmid cloning of Rickettsia prowazekii antigens in Escherichia coli K-12.
Rickettsia prowazekii DNA was partially digested with Sau3A or HindIII, ligated with the cosmid vector pHC79, packaged in vitro, and transduced into Escherichia coli HB101. Cosmid cloning of Sau3A-digested rickettsial DNA yielded 1,288 ampicillin-resistant colonies; 798 cosmid clones resulted with H...
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| Udgivet i: | Infect Immun |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC261491/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2981194/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.47.1.157-165.1985 |
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