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A method for the prevention of thrombin-induced degradation of recombinant proteins
A new strategy to prevent degradation of recombinant proteins caused by non-specific cleavage by thrombin is described. We demonstrate that degradation due to non-specific cleavage of recombinant protein mediated by thrombin can be completely prevented by separation of thrombin from the recombinant...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2008
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2614318/ https://ncbi.nlm.nih.gov/pubmed/18279654 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.01.014 |
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