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Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a −80°C Freezer for a Decade

Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT). We report the cloning of a bull using cells retrieved from testicles that had been taken from a dead animal and frozen without cryoprotectant in a −...

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Hlavní autoři: Hoshino, Yoichiro, Hayashi, Noboru, Taniguchi, Shunji, Kobayashi, Naohiko, Sakai, Kenji, Otani, Tsuyoshi, Iritani, Akira, Saeki, Kazuhiro
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2009
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2613553/
https://ncbi.nlm.nih.gov/pubmed/19129919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0004142
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