Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa

This study aimed to improve the staining of frozen-thawed Japanese Black bull sperm acrosomes with fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA). Spermatozoa were washed, fixed with 1–3% paraformaldehyde (PFA) in suspension for 10, 20, and 30 min, permeabilized with 0–2% Triton...

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Detalhes bibliográficos
Publicado no:J Vet Med Sci
Principais autores: RAJABI-TOUSTANI, Reza, AKTER, Quzi Sharmin, ALMADALY, Essam A., HOSHINO, Yoichiro, ADACHI, Hiromichi, MUKOUJIMA, Koushi, MURASE, Tetsuma
Formato: Artigo
Idioma:Inglês
Publicado em: The Japanese Society of Veterinary Science 2019
Assuntos:
Theriogenology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6541845/
https://ncbi.nlm.nih.gov/pubmed/30606905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1292/jvms.18-0560
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