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Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa
This study aimed to improve the staining of frozen-thawed Japanese Black bull sperm acrosomes with fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA). Spermatozoa were washed, fixed with 1–3% paraformaldehyde (PFA) in suspension for 10, 20, and 30 min, permeabilized with 0–2% Triton...
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| Publicado no: | J Vet Med Sci |
|---|---|
| Principais autores: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Japanese Society of Veterinary Science
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6541845/ https://ncbi.nlm.nih.gov/pubmed/30606905 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1292/jvms.18-0560 |
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