Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa

This study aimed to improve the staining of frozen-thawed Japanese Black bull sperm acrosomes with fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA). Spermatozoa were washed, fixed with 1–3% paraformaldehyde (PFA) in suspension for 10, 20, and 30 min, permeabilized with 0–2% Triton...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Udgivet i:J Vet Med Sci
Main Authors: RAJABI-TOUSTANI, Reza, AKTER, Quzi Sharmin, ALMADALY, Essam A., HOSHINO, Yoichiro, ADACHI, Hiromichi, MUKOUJIMA, Koushi, MURASE, Tetsuma
Format: Artigo
Sprog:Inglês
Udgivet: The Japanese Society of Veterinary Science 2019
Fag:
Theriogenology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6541845/
https://ncbi.nlm.nih.gov/pubmed/30606905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1292/jvms.18-0560
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!

Lignende værker