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Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

BACKGROUND: An ideal format to describe transcriptome would be its composition measured on the scale of absolute numbers of individual mRNAs per cell. It would help not only to precisely grasp the structure of the transcriptome but also to accelerate data exchange and integration. RESULTS: We concei...

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Detalhes bibliográficos
Main Authors: Miura, Fumihito, Kawaguchi, Noriko, Yoshida, Mikio, Uematsu, Chihiro, Kito, Keiji, Sakaki, Yoshiyuki, Ito, Takashi
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2612024/
https://ncbi.nlm.nih.gov/pubmed/19040753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-9-574
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