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Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

BACKGROUND: An ideal format to describe transcriptome would be its composition measured on the scale of absolute numbers of individual mRNAs per cell. It would help not only to precisely grasp the structure of the transcriptome but also to accelerate data exchange and integration. RESULTS: We concei...

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Hlavní autoři: Miura, Fumihito, Kawaguchi, Noriko, Yoshida, Mikio, Uematsu, Chihiro, Kito, Keiji, Sakaki, Yoshiyuki, Ito, Takashi
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2612024/
https://ncbi.nlm.nih.gov/pubmed/19040753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-9-574
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