Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli.

A gene bank of Mycobacterium bovis BCG DNA in Escherichia coli was constructed by cloning Sau3A-cleaved mycobacterium DNA fragments into the lambda vector EMBL3. The expression of mycobacterial antigens was analyzed by Western blotting with hyperimmune rabbit sera. Among 770 clones tested, several w...

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Veröffentlicht in:	Infect Immun
Hauptverfasser:	Thole, J E, Dauwerse, H G, Das, P K, Groothuis, D G, Schouls, L M, van Embden, J D
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1985
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC261151/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3905615/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.50.3.800-806.1985
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