Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli.

A gene bank of Mycobacterium bovis BCG DNA in Escherichia coli was constructed by cloning Sau3A-cleaved mycobacterium DNA fragments into the lambda vector EMBL3. The expression of mycobacterial antigens was analyzed by Western blotting with hyperimmune rabbit sera. Among 770 clones tested, several w...

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Bibliografiska uppgifter
Huvudupphovsmän: Thole, J E, Dauwerse, H G, Das, P K, Groothuis, D G, Schouls, L M, van Embden, J D
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1985
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC261151/
https://ncbi.nlm.nih.gov/pubmed/3905615
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