Cloning and expression of the leukotoxin gene of Pasteurella haemolytica A1 in Escherichia coli K-12.
A clone bank of Pasteurella haemolytica A1 was constructed by partial digestion of the genomic DNA with Sau3A and ligation of 5- to 10-kilobase-pair fragments into the BamHI site of the plasmid vector pBR322. After transformation into Escherichia coli K-12, a total of 4 X 10(3) recombinant clones wa...
Gardado en:
| Publicado en: | Infect Immun |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1985
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC261130/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3905610/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.50.3.667-671.1985 |
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