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Cloning and expression of the leukotoxin gene of Pasteurella haemolytica A1 in Escherichia coli K-12.

A clone bank of Pasteurella haemolytica A1 was constructed by partial digestion of the genomic DNA with Sau3A and ligation of 5- to 10-kilobase-pair fragments into the BamHI site of the plasmid vector pBR322. After transformation into Escherichia coli K-12, a total of 4 X 10(3) recombinant clones wa...

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Autors principals: Lo, R Y, Shewen, P E, Strathdee, C A, Greer, C N
Format: Artigo
Idioma:Inglês
Publicat: 1985
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC261130/
https://ncbi.nlm.nih.gov/pubmed/3905610
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