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A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK(1) cells expressing aquaporin-2
Vasopressin (VP)-induced exocytosis was dissected in native and aquaporin-2 (AQP2)-expressing renal LLC-PK(1) cells by a fluorimetric exocytosis assay based on soluble secreted yellow fluorescent protein (ssYFP). YFP was targeted to the secretory pathway by addition of an 18-amino acid signal peptid...
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| Hoofdauteurs: | , , , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
American Physiological Society
2008
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2603565/ https://ncbi.nlm.nih.gov/pubmed/18799651 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajpcell.00344.2008 |
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