A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK(1) cells expressing aquaporin-2

Vasopressin (VP)-induced exocytosis was dissected in native and aquaporin-2 (AQP2)-expressing renal LLC-PK(1) cells by a fluorimetric exocytosis assay based on soluble secreted yellow fluorescent protein (ssYFP). YFP was targeted to the secretory pathway by addition of an 18-amino acid signal peptid...

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Detalhes bibliográficos
Main Authors: Nunes, Paula, Hasler, Udo, McKee, Mary, Lu, Hua A. J., Bouley, Richard, Brown, Dennis
Formato: Artigo
Idioma:Inglês
Publicado em: American Physiological Society 2008
Assuntos:
Methods in Cell Physiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2603565/
https://ncbi.nlm.nih.gov/pubmed/18799651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajpcell.00344.2008
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