Bordetella pertussis adenylate cyclase: isolation and purification by calmodulin-sepharose 4B chromatography.
Purified preparations of adenylate cyclase were obtained from crude urea extracts of Bordetella pertussis by a one-step calmodulin affinity chromatography technique. Diluted extract was loaded onto the column and washed, and adenylate cyclase was eluted with 10mM EGTA [ethylene glycol-bis(beta-amino...
Enregistré dans:
| Publié dans: | Infect Immun |
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| Auteur principal: | |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1987
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260290/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2878883/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.1.129-134.1987 |
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