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Bordetella pertussis adenylate cyclase: isolation and purification by calmodulin-sepharose 4B chromatography.

Purified preparations of adenylate cyclase were obtained from crude urea extracts of Bordetella pertussis by a one-step calmodulin affinity chromatography technique. Diluted extract was loaded onto the column and washed, and adenylate cyclase was eluted with 10mM EGTA [ethylene glycol-bis(beta-amino...

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Библиографические подробности
Главный автор: Friedman, R L
Формат: Artigo
Язык:Inglês
Опубликовано: 1987
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC260290/
https://ncbi.nlm.nih.gov/pubmed/2878883
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