Isolation, characterization, and mapping of Tn5 insertions into the 140-megadalton invasion plasmid defective in the mouse Sereny test in Shigella flexneri 2a.

Using Shigella flexneri 2a YSH6000, we isolated 304 independent Tn5 insertion mutants in the 230-kilobase invasion plasmid, pMYSH6000. The site of each Tn5 insertion was assigned to 23 SalI fragments on the previously made SalI cleavage map of pMYSH6000. Among the 304 insertions, 150 were negative i...

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Publicado en:	Infect Immun
Autores principales:	Sasakawa, C, Makino, S, Kamata, K, Yoshikawa, M
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1986
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260112/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3019891/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.54.1.32-36.1986
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