Isolation, characterization, and mapping of Tn5 insertions into the 140-megadalton invasion plasmid defective in the mouse Sereny test in Shigella flexneri 2a.

Using Shigella flexneri 2a YSH6000, we isolated 304 independent Tn5 insertion mutants in the 230-kilobase invasion plasmid, pMYSH6000. The site of each Tn5 insertion was assigned to 23 SalI fragments on the previously made SalI cleavage map of pMYSH6000. Among the 304 insertions, 150 were negative i...

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Detalles Bibliográficos
Main Authors: Sasakawa, C, Makino, S, Kamata, K, Yoshikawa, M
Formato: Artigo
Idioma:Inglês
Publicado: 1986
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC260112/
https://ncbi.nlm.nih.gov/pubmed/3019891
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