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Molecular analysis of the M protein of Streptococcus equi and cloning and expression of the M protein gene in Escherichia coli.

A Streptococcus equi gene bank was constructed in the bacteriophage lambda gt11 cloning vector, and hybrid phage plaques were screened with S. equi M protein antiserum. A hybrid phage expressing the S. equi M protein (lambda gt11/SEM7) was identified and lysogenized into Escherichia coli Y1089. The...

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Détails bibliographiques
Publié dans:Infect Immun
Auteurs principaux: Galán, J E, Timoney, J F
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1987
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260046/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316035/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.12.3181-3187.1987
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