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An engineered nonsense URA3 allele provides a versatile system to detect the presence, absence and appearance of the [PSI(+)] prion in Saccharomyces cerevisiae
Common methods to identify yeast cells containing the prion form of the Sup35 translation termination factor, [PSI(+)], involve a nonsense suppressor phenotype. Decreased function of Sup35p in [PSI(+)] cells leads to readthrough of certain nonsense mutations in a few auxotrophic markers, for example...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2006
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2600413/ https://ncbi.nlm.nih.gov/pubmed/16491470 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.1341 |
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