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A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4.

The emm6.1 allele of Streptococcus pyogenes JRS4 was deleted by using the conjugative transposon Tn916. The aphA-3 gene, conferring resistance to kanamycin, was cloned between the sequences flanking the structural gene for the type 6 M protein (emm6.1) and inserted into the BstXI site of Tn916 to ge...

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Hlavní autoři: Norgren, M, Caparon, M G, Scott, J R
Médium: Artigo
Jazyk:Inglês
Vydáno: 1989
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC259915/
https://ncbi.nlm.nih.gov/pubmed/2553613
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