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2D-measurement of proton T(1ρ) relaxation in unlabeled proteins: Mobility changes in α-bungarotoxin upon binding of an acetylcholine receptor peptide.

A method for the measurement of proton T(1ρ) relaxation times in unlabeled proteins is described using a variable spin-lock pulse after the initial non-selective 90° excitation in a HOHAHA pulse-sequence. The experiment is applied to α-bungarotoxin (α-BTX) and its complex with a 25-residue peptide d...

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Detalhes bibliográficos
Main Authors: Samson, Abraham O., Chill, Jordan H., Anglister, Jacob
Formato: Artigo
Idioma:Inglês
Publicado em: 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2597414/
https://ncbi.nlm.nih.gov/pubmed/16086595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi050645h
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