Affinity purification and characterization of Shiga-like toxin II and production of toxin-specific monoclonal antibodies.

Shiga-like toxin (SLT-II) was purified to apparent homogeneity from Escherichia coli K-12 strain NM522 containing the cloned toxin genes on recombinant plasmid pEB1. Purification was accomplished by a series of column chromatography techniques: anion-exchange, chromatofocusing, cation-exchange, and...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Downes, F P, Barrett, T J, Green, J H, Aloisio, C H, Spika, J S, Strockbine, N A, Wachsmuth, I K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC259503/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3294179/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.56.8.1926-1933.1988
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!