Affinity purification and characterization of Shiga-like toxin II and production of toxin-specific monoclonal antibodies.

Shiga-like toxin (SLT-II) was purified to apparent homogeneity from Escherichia coli K-12 strain NM522 containing the cloned toxin genes on recombinant plasmid pEB1. Purification was accomplished by a series of column chromatography techniques: anion-exchange, chromatofocusing, cation-exchange, and...

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Bibliografiska uppgifter

I publikationen:	Infect Immun
Huvudupphov:	Downes, F P, Barrett, T J, Green, J H, Aloisio, C H, Spika, J S, Strockbine, N A, Wachsmuth, I K
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	American Society for Microbiology (ASM) 1988
Ämnen:	Research Article
Länkar:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC259503/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3294179/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.56.8.1926-1933.1988
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