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Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of isocitrate dehydrogenase 2 (Rv0066c) from Mycobacterium tuberculosis

Isocitrate dehydrogenase 2 (Icd-2, Rv0066c) from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected and reduced to 3.25 Å resolution in...

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Detalhes bibliográficos
Main Authors: Hatzopoulos, Georgios N., Kefala, Georgia, Mueller-Dieckmann, Jochen
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2593698/
https://ncbi.nlm.nih.gov/pubmed/19052369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108035343
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