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Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase

The MCM gene from the archaeon Halobacterium, with and without its intein, was cloned into an Escherichia coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the inte...

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Bibliografske podrobnosti
Main Authors: Sakakibara, Nozomi, Han, Mimi, Rollor, Claire R, Gilson, Rebecca C, Busch, Courtney, Heo, Gunyoung, Kelman, Zvi
Format: Artigo
Jezik:Inglês
Izdano: Bentham Science Publishers Ltd 2008
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2593048/
https://ncbi.nlm.nih.gov/pubmed/19088906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2174/1874285800802010013
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