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Protein separation and characterization by np-RP-HPLC followed by intact MALDI-TOF mass spectrometry and peptide mass mapping analyses

Due to their complexity, the separation of intact proteins from complex mixtures is an important step to comparative proteomics and the identification and characterization of the proteins by mass spectrometry (MS). In the study reported, we evaluated the use of non-porous-reversed-phase (np-RP) HPLC...

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Detalhes bibliográficos
Main Authors: Dauly, Claire, Perlman, David H., Costello, Catherine E., McComb, Mark E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2590652/
https://ncbi.nlm.nih.gov/pubmed/16823977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr060108z
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