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Deletion of the BCSP31 gene of Brucella abortus by replacement.

The 31-kDa salt-extractable immunogenic protein, BCSP31, was deleted from several Brucella abortus strains by replacement with a marker gene encoding resistance to the antibiotics kanamycin and neomycin. The BCSP31 gene replacement plasmids, constructed with ColE1-derived vectors, were introduced by...

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Autors principals: Halling, S M, Detilleux, P G, Tatum, F M, Judge, B A, Mayfield, J E
Format: Artigo
Idioma:Inglês
Publicat: 1991
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC258969/
https://ncbi.nlm.nih.gov/pubmed/1937745
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