Proteolysis of Clostridium perfringens type A enterotoxin during purification.
The small satellite bands of enterotoxin frequently seen in polyacrylamide gels following purification of Clostridium perfringens enterotoxin were found to be due to endogenous protease activity and were not present if phenylmethylsulfonyl fluoride (PMSF; 1 mM) and EDTA (10 mM) were used in the puri...
Enregistré dans:
| Publié dans: | Infect Immun |
|---|---|
| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1990
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC258757/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2111289/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.58.6.1999-2001.1990 |
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