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Highly l and d enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method

A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. Its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward...

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Bibliografske podrobnosti
Main Authors: Antipov, Eugene, Cho, Art E., Wittrup, K. Dane, Klibanov, Alexander M.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2008
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2584688/
https://ncbi.nlm.nih.gov/pubmed/19004779
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0809851105
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