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Highly l and d enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. Its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2584688/ https://ncbi.nlm.nih.gov/pubmed/19004779 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0809851105 |
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