Effective Mutagenesis of Vibrio fischeri by Using Hyperactive Mini-Tn5 Derivatives

We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of ∼10(−6). This simp...

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Autors principals: Lyell, Noreen L., Dunn, Anne K., Bose, Jeffrey L., Vescovi, Susan L., Stabb, Eric V.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2008
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Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583470/
https://ncbi.nlm.nih.gov/pubmed/18805998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01330-08
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