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Effective Mutagenesis of Vibrio fischeri by Using Hyperactive Mini-Tn5 Derivatives
We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of ∼10(−6). This simp...
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| Hlavní autoři: | , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
2008
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2583470/ https://ncbi.nlm.nih.gov/pubmed/18805998 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01330-08 |
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